| .姜黄素对人肝L-02细胞胆固醇合成及转运蛋白表达的影响[J].,2011,13(3):. |
| 姜黄素对人肝L-02细胞胆固醇合成及转运蛋白表达的影响 |
| Effects of Curcumin on Cholesterol Synthesis and Transport Protein Expression in human L-02 hepatocyte |
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| DOI： |
| 中文关键词: 姜黄素 人肝L-02细胞 胆固醇合成 转运蛋白 HMG-CoA还原酶 Caveolin-1 |
| 英文关键词: Curcumin Human L-02 hepatocyte Cholesterol systhesis Transport protein HMG-CoA reductase Caveolin-1 |
| 基金项目: |
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| 摘要点击次数: 1575 |
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| 中文摘要: |
| 目的以正常人肝L-02细胞建立的脂肪变性肝细胞模型为研究对象,观察姜黄素对肝细胞胆固醇合成及转运的影响。方法用MTT法观察姜黄素对脂肪变性人肝L-02细胞模型增殖的影响,用油红O染色定性观察细胞内脂滴形成情况;逆转录-多聚酶链反应(RT-PCR)半定量检测胆固醇合成限速酶HMG-CoA还原酶及转运蛋白Caveolin-1在mRNA水平的表达;Western-blotting法检测细胞caveolin-1的表达;高效液相色谱法(HPLC)定量检测细胞内外胆固醇各组分含量。结果姜黄素呈浓度和时间依赖性减少细胞内脂滴数量,降低脂变肝细胞内总胆固醇(TC)、胆固醇酯(CE)含量,增加培养基中游离胆固醇(FC)含量,同时减少HMG-CoA还原酶的表达,增加Caveolin-1 mRNA的表达,并且呈浓度时间依赖性增加caveolin-1的蛋白表达。结论姜黄素降低胆固醇作用可能与Caveolin-1的上调、HMG-CoA还原酶的下调有关。 |
| 英文摘要: |
| Objective Objective To investigate the fatty changed human L-02 hepatocyte under the control of curcumin,to observe the effects of curcumin on cholesterol synthesis and transport.Methods MTT-assay was used to observe the inhibitory effect of curcumin on cell growth of fatty changed human liver L-02 cells;observed the formation of lipid droplets in the cells under oil red O dye.detect the HMG-CoA reductase and caveolin-1 mRNA's expression and protein expression of the hepatocytes of each group by reverse tra... |
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